{"id":12337,"date":"2016-11-21T07:45:39","date_gmt":"2016-11-21T10:45:39","guid":{"rendered":"https:\/\/www.infobioquimica.com\/new\/?p=12337"},"modified":"2016-11-21T07:45:39","modified_gmt":"2016-11-21T10:45:39","slug":"imaging-technique-reveals-movement-of-genetic-material-within-nucleus","status":"publish","type":"post","link":"https:\/\/www.infobioquimica.com\/new\/2016\/11\/21\/imaging-technique-reveals-movement-of-genetic-material-within-nucleus\/","title":{"rendered":"Imaging technique reveals movement of genetic material within nucleus"},"content":{"rendered":"<p>Researchers used powerful X-ray microscope at Berkeley Lab&#8217;s Advanced Light Source (ALS) to study differentiating neurons as they mature. Using X-rays, images were taken from many angles to create a three-dimensional composite image from two-dimensional images, showing the changing structure of chromatin during the maturation of olfactory neurons of mice.<\/p>\n<p><iframe loading=\"lazy\" width=\"696\" height=\"522\" src=\"https:\/\/www.youtube.com\/embed\/YstKOinbY3Y?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>Chromatin functions to package DNA so that it can fit inside of a cell, and forms chromosomes. In the video above, take a trip through the nucleus of a cell; in the video below, DNA reorganization in the nucleus a mouse cell is illustrated.<\/p>\n<p><iframe loading=\"lazy\" width=\"696\" height=\"392\" src=\"https:\/\/www.youtube.com\/embed\/a94Sb2b2xTY?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>The investigators were also able to take measurements of the packing inside of a type of chromatin, heterochromatin, and revealed more about the role of a protein that aids in the packaging of heterochromatin, and its localization to the nucleus.<\/p>\n<p>\u00abIt&#8217;s a new way of looking at the nucleus where we don&#8217;t have to chemically treat the cell,\u00bb explained Carolyn Larabell, the Director of the National Center for X-ray Tomography (NCXT), a collaboration of Berkeley Lab and UC San Francisco (UCSF). \u00abBeing able to directly image and quantify changes in the nucleus is enormously important and has been on cell biologists&#8217; wish list for many years.\u00bb<\/p>\n<p>She added that Chromatin is \u00abnotoriously sensitive\u00bb to chemical stains and additives frequently used in biological imaging protocols that highlight regions of interest in a sample. \u00abUntil now, it has only been possible to image the nucleus indirectly by staining it, in which case the researcher has to take a leap of faith that the stain was evenly distributed.\u00bb<\/p>\n<p>Larabell, who is also a faculty scientist at Berkeley Lab and a UCSF professor, explained that it had been thought that chromatin existed as a group of disconnected islands, but the latest report indicated that chromatin is compartmentalized into two areas of \u00abcrowding\u00bb that comprise a continuous network throughout the nucleus.<\/p>\n<p><strong>Source:<\/strong> <a href=\"http:\/\/www.labroots.com\/trending\/microbiology\/4609\/imaging-technique-reveals-movement-genetic-material-nucleus?utm_content=buffer57163&amp;utm_medium=social&amp;utm_source=facebook.com&amp;utm_campaign=buffer\" target=\"_blank\">LabRoots<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Researchers used powerful X-ray microscope at Berkeley Lab&#8217;s Advanced Light Source (ALS) to study differentiating neurons as they mature. Using X-rays, images were taken from many angles to create a three-dimensional composite image from two-dimensional images, showing the changing structure of chromatin during the maturation of olfactory neurons of mice. Chromatin functions to package DNA [&hellip;]<\/p>\n","protected":false},"author":2620,"featured_media":12338,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"twitterCardType":"summary_large_image","cardImageID":0,"cardImage":"","cardTitle":"","cardDesc":"","cardImageAlt":"","cardPlayer":"","cardPlayerWidth":0,"cardPlayerHeight":0,"cardPlayerStream":"","cardPlayerCodec":"","footnotes":""},"categories":[655],"tags":[17792,17786,17790,6903,17788,5990,17784,17782],"class_list":["post-12337","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-noticias-ingles","tag-carolyn-larabell","tag-chromatin","tag-chromosomes","tag-dna-en","tag-heterochromatin","tag-neuron","tag-neurons","tag-x-ray-microscope"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/posts\/12337","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/users\/2620"}],"replies":[{"embeddable":true,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/comments?post=12337"}],"version-history":[{"count":1,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/posts\/12337\/revisions"}],"predecessor-version":[{"id":12339,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/posts\/12337\/revisions\/12339"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/media\/12338"}],"wp:attachment":[{"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/media?parent=12337"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/categories?post=12337"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.infobioquimica.com\/new\/wp-json\/wp\/v2\/tags?post=12337"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}